PrP
Normal development
Resistance to scrapie
| Line Name | DNA Construct | |
|---|---|---|
| 1. | Prn-p(0/+)/SHaPrP(0 | |
| 2. | Prn-p(0/0)/SHaPrP(0 | |
| 3. | Tg(MHM2)-285 | Chimeric construct MHM2 PrP has been previously described (Scott et. al. 1992) and contains two amino acid substitutions from Syrian hamsters. Recombinant expression ca.... |
| 4. | Tg(MHM2)-321 | Chimeric construct MHM2 PrP has been previously described (Scott et. al. 1992) and contains two amino acid substitutions from Syrian hamsters. Recombinant expression ca.... |
| 5. | Tg(MHM2)-294 | Chimeric construct MHM2 PrP has been previously described (Scott et. al. 1992) and contains two amino acid substitutions from Syrian hamsters. Recombinant expression ca.... |
| 6. | Tg(MH2M)-92 | The MH2M chimeric gene was constructed by exchanging the Kpnl- |
| 7. | Tg HaPrP #69 | The HaPrP gene is encoded by two exons: one encodes a 5' untranslated leader of ~70 nucleotides, and a second 2.0 kb exon contains the open reading frame (ORF). A hams.... |
| 8. | Tg HaPrP #7 | The HaPrP gene is encoded by two exons: one encodes a 5' untranslated leader of ~70 nucleotides, and a second 2.0 kb exon contains the open reading frame (ORF). A hams.... |
| 9. | Tg HaPrP #81 | The HaPrP gene is encoded by two exons: one encodes a 5' untranslated leader of ~70 nucleotides, and a second 2.0 kb exon contains the open reading frame (ORF). A hams.... |
| 10. | Tg HaPrP #71 | The HaPrP gene is encoded by two exons: one encodes a 5' untranslated leader of ~70 nucleotides, and a second 2.0 kb exon contains the open reading frame (ORF). A hams.... |
| 11. | Tg HaPrP #20 | The HaPrP gene is encoded by two exons: one encodes a 5' untranslated leader of ~70 nucleotides, and a second 2.0 kb exon contains the open reading frame (ORF). A hams.... |
| 12. | Prn-p (+/-) | pUC18 containing an 8.9-kb BamHl fragment of mouse Prn-p genomic sequences including the PrP-encoding exon 3. The 4.2-kb BamHl-EcoRl fragment was transferred to Bluescr.... |
| 13. | Prn-p(0/0) | One Prn-p allele of murine embryonic stem cells was disrupted by homologous recombination with a 4.8 kb DNA fragment in which codons 4 to 187 of the 254-codon open readi.... |
| 14. | Prn-p (-/-) | pUC18 containing an 8.9-kb BamHl fragment of mouse Prn-p genomic sequences including the PrP-encoding exon 3. The 4.2-kb BamHl-EcoRl fragment was transferred to Bluescr.... |
