Van Kaer L, Ashton-Rickardt PG, Ploegh HL, Tonegawa S:
TAP1 mutant mice are deficient in antigen presentation, surface class I molecules, and CD4-8+ T cells.
Cell 1992 Dec 24;71(7):1205-14
ABSTRACT
The transporter associated with the antigen processing 1 (TAP1) gene
encodes a subunit for a transporter, presumed to be involved in the
delivery of peptides across the endoplasmic reticulum membrane to
class I molecules. We have generated mice with a disrupted TAP1 gene
using embryonic stem cell technology. TAP1-deficient mice are
defective in the stable assembly and intracellular transport of
class I molecules and consequently show severely reduced levels of
surface class I molecules. These properties are strikingly similar
to those described for the TAP2 mutant cell line RMA-S. Cells from
the TAP1-deficient mice are unable to present cytosolic antigens to
class I-restricted cytotoxic T cells. As predicted from the near
absence of class I surface expression, TAP1-deficient mice lack
CD4-8+ T cells.
