[Frontiers in Bioscience 8, d603-619, May 1, 2003]

RETROVIRAL TECHNOLOGY - APPLICATIONS FOR EXPRESSED PEPTIDE LIBRARIES

Roland Wolkowicz, and Garry P. Nolan

Department of Microbiology and Immunology, Baxter Laboratory in Genetic Pharmacology, Stanford University Medical Center, Stanford, California

TABLE OF CONTENTS

1. Abstract
2. Introduction
3. In the beginning: The first phage display
4. The philosophy behind libraries: from cDNA to peptides
5. Current application of peptide phage display libraries
6. A matter of shape ― constrained and linear
7. Retroviruses as a means to deliver genetic information for genetic screens in mammalian cells
8. Retrovirus production from retroviral vectors- a tutorial
9. Construction of the peptide library―a tutorial
10. Retroviral expression peptide libraries: from infection to rescue
11. Current application of retroviral cDNA and peptide libraries
12. Peptide libraries―associations with classic and new technologies
13. Perspective
14. References

1. ABSTRACT

The cell is an extremely complex network of interactions between large numbers of molecules. Understanding this entire network and the information arising from it is an overwhelming and challenging task. Reverse genetics has given us the possibility to discover unknown interactions and their related pathways. With the help of peptide libraries, interactions between biomolecules can be disrupted or distorted and the signaling pathways where these proteins are involved, altered. Consequently, novel biological pathways can be discerned.