[Frontiers in Bioscience E3, 65-73, January 1, 2011]

Influence of tissue particles on Fas expression in idiopathic interstitial pneumonia

Yasuo Shimizu1,2, S Matsuzaki1, T Satoh3, T Ohkubo3, A Yokoyama3, Y Ishii3, T Kamiya3, K Arakawa3, K Shimizu K4, Tanaka S5, Mori M1, K Dobashi6

1Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine, Maebashi Gunma Japan, 2WHO Collaborating Center of Prevention and Control of Chronic Respiratory Diseases, Dokkyo University, (DU-WCC), Mibu Tochigi, Japan, 3Japan Atomic Energy Agency, Takasaki Advanced Radiation Research Institute, Takasaki, Gunma, Japan, 4Division of Thoracic and Visceral Organ Surgery, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan,

5Department of General Surgical Science, Gunma University Graduate School of Medicine, Maebashi Gunma, Japan, 6Gunma University Faculty of Health Science, Maebashi Gunma, Japan

TABLE OF CONTENTS

1. Abstract
2. Introduction
2.1. in-air micro-PIXE analysis system
3. Methods
3.1. Patients and sample preparation
3.2. In-air micro-PIXE system
3.3. Immunohistochemistry
3.4. Calculation of the content of elements
3.5. Statistical analysis
4. Results <
4.1. Detection of particles
4.2. Quantitative analysis of Si and Fe and the co-localization of particles with Fas expressions
5. Discussion
5.1. Si and Fe were detected both in IIP and non-IIP lung tisuues
5.2. Co-localization of particle depositions and Fas expressions in lung of IIP patients
5.3. Fe deposition in lung tissue
5.4. Limitations of this study
6. Acknowledgements
7. References

1. ABSTRACT

Idiopathic interstitial pneumonia (IIP) is a progressive fibrosing interstitial pneumonia of unknown etiology with a poor prognosis. The aim of this study is to prove the occurrence of particle deposition and particle-induced tissue damage in IIP by examining proapoptotic Fas expression with in-air microparticle induced X-ray emission (in-air micro-PIXE) analysis. A total of 21 patients were enrolled. Lung tissues from 12 IIP patients and nontumorous lung tissues from 9 lung cancer patients (as a control) were subjected to in-air micro-PIXE analysis. The distribution of particles in lung tissue was compared with the localization of Fas expression by immunohistochemistry. Silicon (Si) was identified in 58.3% of IIP samples and 44.4% of control samples. Iron (Fe) was identified 25% in IIP samples and 11.1% in control samples. The mean lung tissue content of Si and Fe relative to S did not differ between IIP and control patients. Only two IIP patients showed the co-localization of Si and Fe deposition with Fas expression. Adaptation of this method would contribute to assess the influence of particles on IIP.