[Frontiers in Bioscience E3, 256-263, January 1, 2011]

Isolation and expansion of adipose-derived stem cells for tissue engineering

Trine Fink1, Jeppe Grondahl Rasmussen1,2, Pia Lund1, Linda Pilgaard1, Kjeld Soballe3, Vladimir Zachar1

1Laboratory for Stem Cell Research, Department of Health Science and Technology, Aalborg University, Denmark, 2Department of Pharmacology, Aarhus University, Denmark, 3Orthopaedic Research Laboratory, Aarhus University Hospital, Denmark

TABLES

Table 1. Cell doubling time (Td) as a function of initial cell plating density

Plating density (cells/cm2)

Doubling time (Td)

50

3.21

100

1.45

200

1.51

800

3.18