[Frontiers in Bioscience E3, 355-363, January 1, 2011]

Spatiotemporal expression of D10Wsu52e in the developing mouse pancreas

Jiangdong Zhao1, Xiaofang Ju1, Liqiang Zhang1, Yang An1, Zhenwu Zhang1, Zhongwu Sun1, Naizheng Ding2, Chun-Bo Teng1

1Laboratory of Animal Development Biology, College of Life Science, Northeast Forestry University, Hexing Road, Harbin, China, 2 School of Biological Science and Technology, Central South University, Changsha, 410012 China

TABLE OF CONTENTS

1. Abstract
2. Introduction
3. Materials and methods
3.1. Animals
3.2. RNA isolation and reverse transcription
3.3. Real-time quantitative PCR of D10Wsu52e mRNA
3.4. Preparation of probe and in situ hybridization
3.5. Histochemistry
4. Results
4.1. Expression of D10Wsu52e mRNA in the whole developing embryos
4.2. Expression of D10Wsu52e mRNA in the developing mouse pancreas
4.3. Expression of paxillin and vinculin mRNAs in the developing mouse pancreas
5. Discussion
6. Acknowledgements
7. References

1. ABSTRACT

D10Wsu52e is a recently discovered and highly conserved mouse gene. FAAP, the protein encoded by D10Wsu52e, participates in regulation of integrin-based focal adhesions. To explore the function of FAAP in pancreas development, we assessed the spatiotemporal expression of D10Wsu52e, paxillin and vinculin in the developing mouse pancreas through quantitative RT-PCR, in situ hybridization and histochemistry methods. Our results show that, at e9.5 and e10.5, D10Wsu52e mRNA is mainly expressed in the brain, optic vesicles, otic vesicles, limb buds, somites and gut, and also in the pancreatic buds at e10.5. Subsequently, D10Wsu52e mRNA is expressed mainly in the epithelial progenitors at e12.5, then decreases in the endocrine precursors and ductal cells, whereas still maintains in the exocrine precursors until e15.5. At e17.5, D10Wsu52e mRNA is highly expressed in exocrine acinar cells, but weakly in ductal and endocrine cells. Furthermore, the expression patterns of paxillin and vinculin mRNAs are similar to D10Wsu52e from e12.5 to e15.5, which suggests that D10Wsu52e may be related to the acinar development, adhesion and migration of progenitors and endocrine precursors.