[Frontiers in Bioscience S3, 1047-1057, June 1, 2011]

In vitro studies of early cardiac remodeling: impact on contraction and calcium handling

Kaylan M. Haizlip, Paul M.L. Janssen

Department of Physiology and Cell Biology and D. Davis Heart Lung Institute, College of Medicine, The Ohio State University, 1645 Neil Avenue, Columbus, OH 43210 USA

TABLE OF CONTENTS

1. Abstract
2. Introduction
3.1. Current experimental model systems
3.2. Excitation
3.3. Myofilaments
3.4. Calcium Transient Decline
3.5. Recent developments in multicellular tissues for investigation of remodeling and calcium handling
4. Conclusions
5. Acknowledgements
6. References

1. ABSTRACT

Cardiac remodeling, hypertrophy, and alterations in calcium signaling are changes of the heart that often lead to failure. After a hypertrophic stimulus, the heart progresses through a state ofcompensated hypertrophy which over time leads to decompensated hypertrophy or failure. It is at this point that a cardiac transplant is required for survival making early detection imperative. Current experimental systems used to study the remodeling of the heart include in vivo systems (the whole body), isolated organ and sub-organ tissue, and the individual cardiac muscle cells and organelles.. During pathological remodeling there is a derangement in the intracellular calcium handling processes. These derangements are thought to lead to a dysregulation of contractile output. Hence, understanding the mechanism between remodeling and dysregulation is of great interest in the cardiac field and will ultimately help in the development of future treatment and early detection. This review will center on changes in contraction and calcium handling in early cardiac remodeling, with a specific focus on findings in two different in vitro model systems: multicellular and individual cell preparations.