[Frontiers in Bioscience E4, 1255-1262, January 1, 2012]

BNP and iNOS in decompensated chronic heart failure: a linear correlation

Lorenza Speranza1, Sara Franceschelli1, Graziano Riccioni2, Marta Di Nicola3, Benedetta Ruggeri4, Sabina Gallina1,4, Mario Felaco1, Alfredo Grilli1

1Department of Human Movement Sciences ,University G. D'Annunzio, Chieti Italy, 2Cardiology Unit, San Camillo de Lellis Hospital, Manfredonia, Foggia, Italy, 3Department of Biomedical Sciences University G. D'Annunzio, Chieti Italy, 4Cardiology Department SS Annunziata Hospital, Via dei Vestini-Chieti Italy


1. Abstract
2. Introduction
3. Patients and methods
3.1. Study population
4. Laboratory procedures
4.1. Natriuretic Peptide
4.2. Echocardiographic-Doppler evaluation
4.3. Cytosol preparation
4.4. Citrulline synthesis
4.5. Measurement of nitrite levels
4.6. Detection of iNOS and 3-nitrotyrosine
5. Statistical analysis
6. Results
6.1. The demographic, clinical and echocardiographic characteristics
6.2. Expression of iNOS and 3-nitrotyrosine proteins
6.3. NO production (activity of iNOS)
6.4. Nitrite levels
6.5. Natriuretic peptide
6.6. Correlations between BNP level and iNOS activity
7. Discussion
8. Acknowledgement
9. References


Heart Failure (HF) is characterized by activation inflammatory mediators that contributes to the disease progression. Brain natriuretic peptide (BNP) plasma levels increase in HF with a prognostic significance. The aim of this study was to evaluate the expression and activity of inducible nitric oxide synthase (iNOS) in peripheral blood mononuclear cell (PBMC) extracted from patients and a possible linear correlation between iNOS and plasma levels of BNP in decompensated chronic HF (DCHF) patients. To establish the DCHF, thirty-five male patients were evaluated. All patients were venesected within 24 h of admission to exclude an inflammatory state through evaluation of c-reactive protein. Only twenty subjects showed symptoms of DCHF were included in the study. Other patients were included in the control group. In DCHF, left ventricular ejection fraction (LVEF) % was reduced and systolic pulmonary artery pressure (PAPs) was increased. Furthermore, iNOS expression and BNP plasma levels were significantly higher in patients with DCHF as compared to controls group. These findings indicate that in DCHF patients, iNOS activity exhibits a significant linear correlation with plasmatic BNP level.