[Frontiers in Bioscience E4, 2734-2744, June 1, 2012]

Gene expression in visceral and subcutaneous adipose tissue in overweight women

Mirko Korsic1, Kristina Gotovac2, Matea Nikolac3, Tina Dusek1, Mate Skegro4, Dorotea Muck-Seler3, Fran Borovecki2, Nela Pivac3

1Department of Endocrinology and Metabolic Disorders, Clinical Hospital Center Zagreb, University Hospital Center Zagreb, Zagreb, Croatia, 2Department for Functional Genomics, Center for Translational and Clinical Research, University of Zagreb School of Medicine, and University Hospital Center Zagreb, Zagreb, Croatia, 3Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia, 4Department of Hepato-Biliar Surgery and Transplantation of Abdominal Organs, Clinical Hospital Center Zagreb, University Hospital Center Zagreb, Zagreb, Croatia

TABLE OF CONTENTS

1. Abstract
2. Introduction
3. Materials and methods
3.1. Patients
3.2. Gene expression profiling of subcutaneous and visceral fat tissue
3.3. Gene expression analysis by real-time PCR
3.4. Statistical analysis
4. Results
5. Discussion
6. Acknowledgment
7. References

1. ABSTRACT

Excess weight and obesity are common health problems with multifactorial and polygenic causes. Abdominal or visceral obesity is associated with a higher risk of obesity related complications. The aim of the study was to evaluate differentially expressed genes in visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) of 10 overweight women undergoing elective laparoscopic cholecystectomy. Following expression profiling using microarrays, a set of 294 genes that exhibited differential expression between VAT and SAT was further analyzed to test the functional correlation of gene sets using the Gene Set Enrichment Analysis method. To confirm the functional pathways involved in differential expression between SAT and VAT, additional pathway analysis was done using the GeneGo MetaCore software and the Ingenuity Pathway Analysis. Ten differentially expressed genes were selected according to the microarray data, with seven exhibiting significant differential expression in the RT-PCR experiments. The data from this preliminary study suggest enrichment of inflammation and oxidative stress related pathways in VAT, while insulin homeostasis pathways as well as pathways pertaining to several growth factors are enriched in SAT.