TRIS (hydroxyethyl aminoethane)

mL 0.1 N HCl

Added

Resulting pH

at 23C

Resulting pH

at 37C

5.0

9.10

8.95

7.5

8.92

8.78

10.0

8.74

8.60

12.5

8.62

8.48

15.0

8.50

8.37

17.5

8.40

8.27

20.0

8.32

8.18

22.5

8.23

8.10

25.0

8.14

8.00

27.5

8.05

7.90

30.0

7.96

7.82

32.5

7.87

7.73

35.0

7.77

7.63

37.5

7.66

7.52

40.0

7.54

7.40

42.5

7.36

7.22

45.0

7.20

7.05